rodent decloaker buffer (Biocare Medical)

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Biocare Medical rodent decloaker buffer
Rodent Decloaker Buffer, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rodent+decloaker/decloaker+diva/pmc11852395-77-8-11
Average 86 stars, based on 1 article reviews

rodent decloaker buffer - by Bioz Stars, 2026-10

86/100 stars

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Immunohistochemistry:

Article Title: Site-specific ERα phosphorylation determines sex-dependent metabolic, reproductive, and body-compositional phenotypes in mice
Article Snippet: Stained sections were subsequently dehydrated, cleared by Xylene (Fisher Chemical, X3P-1GAL), and mounted with Micromount (Leica, 3801731). .. For immunohistochemistry, deparaffinized by Xylene (Fisher Chemical, X3P-1GAL) and rehydrated sections underwent antigen retrieval using Rodent Decloaker (Biocare Medical, RD913) and nonspecific binding was minimized using Background Sniper (Biocare Medical). .. Slides were incubated overnight at 4°C with primary antibodies, including anti-PR (1:100, Cell Signaling, C89F7), Ki-67 (1:50, Biocare Medical, CRM325A), anti-ERα (1:1000, Sigma-Aldrich, 06–935) or anti-Rabbit IgG (Biocare Medical, NC495 AA).

Article Title: NAMPT overexpression enhances the regenerative potential of mesenchymal stromal cell-derived extracellular vesicles in experimental AKI.
Article Snippet: .. For immunohistochemistry analysis, formalin-fixed, 3-μm paraffin-embedded kidney sections were incubated for 5 min with Peroxidazed 1 (PX968, Biocare Medical) to quench endogenous peroxidase, after antigen retrieval in Rodent Decloaker (RD913, Biocare Medical). .. After blocking for 30 min with Rodent Block M (RBM961G, Biocare Medical), sections were incubated with the following primary antibodies: rabbit antiNAMPT (1:100; HPA047776, Sigma), rabbit anti-ATP5i (1:100; HPA035010, Sigma), rabbit anti-PGC1α (1:100; ab54481, Abcam), mouse anti-VDAC (1:400; ab186321, Abcam), and rabbit anti-SIRT3 (1:100; ab189860, Abcam) and rat anti-MAC2 antibody (1:600, CL8942AP, Cederlane).

Article Title: NAMPT overexpression enhances the regenerative potential of mesenchymal stromal cell-derived extracellular vesicles in experimental AKI
Article Snippet: .. For immunohistochemistry analysis, formalin-fixed, 3-μm paraffin-embedded kidney sections were incubated for 5 min with Peroxidazed 1 (PX968, Biocare Medical) to quench endogenous peroxidase, after antigen retrieval in Rodent Decloaker (RD913, Biocare Medical). .. After blocking for 30 min with Rodent Block M (RBM961G, Biocare Medical), sections were incubated with the following primary antibodies: rabbit anti-NAMPT (1:100; HPA047776, Sigma), rabbit anti-ATP5i (1:100; HPA035010, Sigma), rabbit anti-PGC1α (1:100; ab54481, Abcam), mouse anti-VDAC (1:400; ab186321, Abcam), and rabbit anti-SIRT3 (1:100; ab189860, Abcam) and rat anti-MAC2 antibody (1:600, CL8942AP, Cederlane).

Binding Assay:

Immunofluorescence:

Article Title: Intermittent Fasting Reduces Obesity-driven Oxidative Stress in the Male Mouse Colon via Changes in Gut Microbiota
Article Snippet: .. Following xylene-based deparaffinization, immunofluorescence heat-induced antigen retrieval was performed at pH 6.0 (Biocare Medical, Rodent Decloaker) and permeabilized for 15 minutes with Triton X-100 0.1% (Millipore). .. The tissue was blocked in 2% bovine serum albumin (Sigma-Aldrich) for 1 hour at room temperature and then incubated at 4°C overnight with primary antibodies against: UEA1 ( L32476 ; Thermo Fisher Scientific), PCNA (ab92552; Abcam, Cambridge, UK), CL-1 (ab15098; Abcam), and CL-3 (ab15102; Abcam).

Incubation:

Article Title: Cannabis Improves Metabolic Dysfunction and Macrophage Signatures in Obese Mice.
Article Snippet: .. Slide sections were incubated with rodent decloaker (Biocare Medical, Cat. No. 272 RD913M) at 95°C for 30 min to ensure antigen retrieval then washed with TBS. ..

Staining:

Article Title: Protein kinase CK2α’ as a dual modulator of immune signaling and synaptic dysfunction in Tauopathy
Article Snippet: .. For DAB staining antigen retrieval was performed using either Tris-EDTA pH=9.0 (Biolegend 422703) or Rodent decloaker (Biocare medical RD913) at 80°C for 30 minutes. ..

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